Overall, what does the dataset suggest about ASBT expression in dogs with CIE compared to controls?

• A. ASBT expression (protein and mRNA) is similar to controls
• B. ASBT is absent in CIE dogs
• C. ASBT expression is markedly higher in CIE
• D. ASBT protein is higher but mRNA lower in CIE

Answer: (A)

The main idea is to determine whether ASBT expression changes in dogs with CIE compared to controls, considering both protein and mRNA levels. The dataset shows that ASBT protein levels and ASBT mRNA levels are similar between the CIE group and controls, meaning there is no upregulation or downregulation detected. This suggests that ASBT-mediated bile acid reabsorption is not altered in these dogs within the study conditions, so ASBT expression does not distinguish CIE from healthy dogs. The other possibilities would require clear differences—absence, marked upregulation, or discordant protein and mRNA levels—which the data do not support.