In the ileum image, what does the red color indicate?

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Study for the ACVIM Small Animal Internal Medicine Exam to enhance your veterinary knowledge. Prepare with flashcards and multiple-choice questions, featuring hints and explanations. Ensure success in your exam journey!

Multiple Choice

In the ileum image, what does the red color indicate?

Explanation:
Red indicates ASBT mRNA expression detected by an mRNA-specific detection method (such as in situ hybridization). The labeled probes bind to the ASBT transcript, producing red signal where the gene is being transcribed in the ileal enterocytes. This pattern looks different from protein staining, which would highlight the transporter itself on the apical membrane; nuclei are usually counterstained a different color (often blue/purple), and mitochondrial markers would require a distinct stain. So the red spots reflect the presence of ASBT mRNA, not the protein or other organelles.

Red indicates ASBT mRNA expression detected by an mRNA-specific detection method (such as in situ hybridization). The labeled probes bind to the ASBT transcript, producing red signal where the gene is being transcribed in the ileal enterocytes. This pattern looks different from protein staining, which would highlight the transporter itself on the apical membrane; nuclei are usually counterstained a different color (often blue/purple), and mitochondrial markers would require a distinct stain. So the red spots reflect the presence of ASBT mRNA, not the protein or other organelles.

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