In the described image, what does the orange color indicate?

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Multiple Choice

In the described image, what does the orange color indicate?

Orange fluorescence comes from the Cy3-labeled probe that is specific for Fusobacterium. In this staining, Fusobacterium is tagged with Cy3 (orange-red), while total bacteria are labeled with FITC (green) and epithelial nuclei (if present) would be a blue counterstain. So the orange signal indicates Fusobacterium attached to the mucosa, not the overall bacterial population or the nuclei. A different probe would be needed to mark Bacteroides, so they wouldn’t appear orange under this labeling.

Orange fluorescence comes from the Cy3-labeled probe that is specific for Fusobacterium. In this staining, Fusobacterium is tagged with Cy3 (orange-red), while total bacteria are labeled with FITC (green) and epithelial nuclei (if present) would be a blue counterstain. So the orange signal indicates Fusobacterium attached to the mucosa, not the overall bacterial population or the nuclei. A different probe would be needed to mark Bacteroides, so they wouldn’t appear orange under this labeling.

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