In histology images, which color is used to indicate ASBT protein?

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Study for the ACVIM Small Animal Internal Medicine Exam to enhance your veterinary knowledge. Prepare with flashcards and multiple-choice questions, featuring hints and explanations. Ensure success in your exam journey!

Multiple Choice

In histology images, which color is used to indicate ASBT protein?

Explanation:
Protein localization in histology is shown by immunohistochemistry, where an antibody binds to the ASBT protein and a chromogen reveals that site with color. The common chromogen, diaminobenzidine (DAB), produces a brown precipitate exactly where the ASBT protein is present, so the brown staining marks the protein’s location. If you were looking at mRNA, that would come from in situ hybridization and show a different color signal; nuclei are typically blue from a hematoxylin counterstain, and the background is non-specific. So brown staining specifically indicates ASBT protein.

Protein localization in histology is shown by immunohistochemistry, where an antibody binds to the ASBT protein and a chromogen reveals that site with color. The common chromogen, diaminobenzidine (DAB), produces a brown precipitate exactly where the ASBT protein is present, so the brown staining marks the protein’s location. If you were looking at mRNA, that would come from in situ hybridization and show a different color signal; nuclei are typically blue from a hematoxylin counterstain, and the background is non-specific. So brown staining specifically indicates ASBT protein.

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